3FCP
Crystal structure of Muconate lactonizing enzyme from Klebsiella pneumoniae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-08-03 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97915 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 79.321, 122.727, 193.536 |
| Unit cell angles | 90.00, 93.04, 90.00 |
Refinement procedure
| Resolution | 24.940 - 1.800 |
| R-factor | 0.229 |
| Rwork | 0.229 |
| R-free | 0.25000 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | Auto-Rickshaw |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 25.000 |
| High resolution limit [Å] | 1.800 |
| Rmerge | 0.087 |
| Number of reflections | 337786 |
| Completeness [%] | 98.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | 30% MPD, 0.1 M Sodium acetate, 0.02 M Calcium chloride , pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |
