3FCD
Crystal Structure of a putative glyoxalase from an environmental bacteria
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-11-07 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 45.630, 37.277, 72.117 |
| Unit cell angles | 90.00, 98.70, 90.00 |
Refinement procedure
| Resolution | 35.640 - 1.920 |
| Rwork | 0.230 |
| R-free | 0.27620 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.500 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELX (& SHARP) |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.085 | 0.282 |
| Number of reflections | 17538 | |
| <I/σ(I)> | 13 | |
| Completeness [%] | 94.5 | 66.1 |
| Redundancy | 9.6 | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 291 | 25% PEG 3350; 0.1M Bis-TRIS; sodium fluoride, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
