3FC7
The crystal structure of a domain of HTR-like protein from Haloarcula marismortui ATCC 43049
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-06-26 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97937, 0.97951 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 55.183, 55.183, 249.917 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 34.540 - 2.650 |
| R-factor | 0.20563 |
| Rwork | 0.202 |
| R-free | 0.27396 |
| Structure solution method | MAD |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.859 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.5.0054) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 2.700 |
| High resolution limit [Å] | 2.650 | 2.650 |
| Rmerge | 0.087 | 0.867 |
| Number of reflections | 7257 | |
| <I/σ(I)> | 29.66 | 2.4 |
| Completeness [%] | 99.1 | 99.4 |
| Redundancy | 6.5 | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 289 | 1.0M Sodium citrate, 0.1M Imidazole, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
