3FBV
Crystal structure of the oligomer formed by the kinase-ribonuclease domain of Ire1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 77 |
Detector technology | CCD |
Collection date | 2008-08-30 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.00000 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 156.820, 163.470, 292.830 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.983 - 3.200 |
R-factor | 0.2374 |
Rwork | 0.235 |
R-free | 0.28350 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2rio |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | ELVES |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 3.310 |
High resolution limit [Å] | 3.200 | 3.200 |
Rmerge | 0.053 | 0.342 |
Number of reflections | 123887 | |
<I/σ(I)> | 16 | 2.2 |
Completeness [%] | 100.0 | 100 |
Redundancy | 23.2 | 23 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 5.6 | 298 | Sodium citrate, pH 5.6, VAPOR DIFFUSION, temperature 298K |