3FBP

STRUCTURE REFINEMENT OF FRUCTOSE-1,6-BISPHOSPHATASE AND ITS FRUCTOSE 2,6-BISPHOSPHATE COMPLEX AT 2.8 ANGSTROMS RESOLUTION

Experimental procedure

Spacegroup name	P 32 2 1
Unit cell lengths	131.600, 131.600, 68.900
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	10.000 * - 2.800
R-factor	0.188 *
Rwork	0.188
RMSD bond length	0.016 *
RMSD bond angle	4.000
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	2.800 *
Rmerge	0.063 *
Total number of observations	61118 *
Number of reflections	15546 *
Completeness [%]	95.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Microdialysis *	7.4 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	enzyme	5-10 (mg/ml)
2	1	2	Tris base	10 (mM)
3	1	2	PEG3350	8 (%(w/v))
4	1	2	EDTA	0.1 (mM)
5	1	2		0.3 (mM)
6	1	2	maleic acid	2.5 (mM)