3FBM
D431N Mutant VP2 Protein of Infectious Bursal Disease Virus; Derived T=1 Particles
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-06-27 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.933 |
| Spacegroup name | P 21 3 |
| Unit cell lengths | 326.725, 326.725, 326.725 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 3.100 |
| R-factor | 0.294 |
| Rwork | 0.294 |
| R-free | 0.29100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2gsy |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.400 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | REFMAC ((Rigid Body)) |
| Refinement software | REFMAC ((Rigid Body)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.270 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Number of reflections | 206393 | |
| <I/σ(I)> | 9.3 | 1.9 |
| Completeness [%] | 99.0 | 95.8 |
| Redundancy | 5.3 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 293 | 14-17% PEG 4000, 0.1M TRIS pH 9.0, 5% isopropanol, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
