3FBL
Crystal structure of ORF132 of the archaeal virus Acidianus Filamentous Virus 1 (AFV1)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | BRUKER AXS MICROSTAR |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2008-01-07 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.54 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 28.353, 47.576, 60.079 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 18.000 - 1.950 |
R-factor | 0.16203 |
Rwork | 0.157 |
R-free | 0.19619 |
Structure solution method | SAD |
RMSD bond length | 0.013 |
RMSD bond angle | 1.066 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHENIX |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.600 | 2.060 |
High resolution limit [Å] | 1.950 | 1.950 |
Rmerge | 0.037 | 0.066 |
Number of reflections | 6349 | |
<I/σ(I)> | 136.6 | 89 |
Completeness [%] | 100.0 | 100 |
Redundancy | 76 | 76 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 12% PEG 8000, 0.025 mM Na2HPO4/KH2PO4, pH 6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |