3FBG
Crystal structure of a putative arginate lyase from Staphylococcus haemolyticus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-11-14 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.97958 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.198, 93.552, 83.240 |
| Unit cell angles | 90.00, 98.86, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.600 |
| R-factor | 0.237 |
| Rwork | 0.235 |
| R-free | 0.27500 |
| Structure solution method | SAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.534 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.2.25) |
| Phasing software | SHELXCD |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 82.246 | 1.690 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.096 | 0.495 |
| Total number of observations | 70391 | |
| Number of reflections | 93896 | |
| <I/σ(I)> | 8.9 | 2.2 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 5.2 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8 | 294 | 100mM Tris HCl pH 8.0, 29% PEG 3350, 200mM magnesium chloride, vapor diffusion, temperature 294K |
