3FB9
The crystal structure of the protein with unknown function from Streptococcus pneumoniae TIGR4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-10-03 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 46.609, 53.118, 72.036 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.760 - 1.800 |
| R-factor | 0.19297 |
| Rwork | 0.191 |
| R-free | 0.23436 |
| Structure solution method | SAD |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.562 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.5.0054) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.760 | 1.846 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.085 | 0.238 |
| Number of reflections | 16247 | |
| <I/σ(I)> | 29.64 | 8.14 |
| Completeness [%] | 99.6 | 97.2 |
| Redundancy | 9.4 | 8.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 289 | 0.2M NaCl, 0.1M Tris, 25%PEG3350, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
