3FB2
Crystal structure of the human brain alpha spectrin repeats 15 and 16. Northeast Structural Genomics Consortium target HR5563a.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4A |
Synchrotron site | NSLS |
Beamline | X4A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-11-11 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.97905 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 65.610, 96.376, 111.145 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 36.410 - 2.300 |
R-factor | 0.219 |
Rwork | 0.219 |
R-free | 0.24800 |
Structure solution method | SAD |
RMSD bond length | 0.006 |
RMSD bond angle | 0.900 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SnB |
Refinement software | CNS (1.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.067 | 0.318 |
Number of reflections | 58903 | |
<I/σ(I)> | 30.8 | 6.03 |
Completeness [%] | 96.6 | 99.2 |
Redundancy | 6 | 5.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | microbatch crystallization under oil | 5 | 277 | 12% PEG 20000, 0.1M magnesium sulfate, 0.1M sodium acetate, pH 5.0, microbatch crystallization under oil, temperature 277K |