3FAY

Crystal structure of the GAP-related domain of IQGAP1

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	CAMD BEAMLINE GCPCC
Synchrotron site	CAMD
Beamline	GCPCC
Temperature [K]	100
Detector technology	CCD
Collection date	2006-08-20
Detector	MAR CCD 165 mm
Wavelength(s)	0.97924, 0.97900, 0.92523
Spacegroup name	P 21 21 2
Unit cell lengths	170.133, 42.115, 59.382
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.000 - 2.200
Rwork	0.228
R-free	0.25820
Structure solution method	MAD
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHELXS
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	2.280
High resolution limit [Å]	2.200	2.200
Number of reflections	20692
<I/σ(I)>	8.9	2.1
Completeness [%]	86.1	38.2
Redundancy	5.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	8.5	277	20% PEG 2000 methyl ether, 500mM MgCl2, 100mM Tris HCL, pH 8.5, VAPOR DIFFUSION, temperature 277K