3FAR
Cation-dependent self-cleavage activity in the duplex form of the subtype-B HIV-1 RNA Dimerization Initiation Site
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS FR591 |
| Temperature [K] | 120 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-04-16 |
| Detector | MAC Science DIP-2000 |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 43.689, 47.541, 57.735 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 7.990 - 2.400 |
| R-factor | 0.239 |
| Rwork | 0.239 |
| R-free | 0.28400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2oiy |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.100 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.430 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Number of reflections | 8591 | |
| <I/σ(I)> | 14.1 | 2.2 |
| Completeness [%] | 94.2 | 94.6 |
| Redundancy | 2.4 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.2 | 310 | MgCl2, Na Cacodylate, KCl, spermine, MPD, pH 6.2, VAPOR DIFFUSION, SITTING DROP, temperature 310K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | MgCl2 | ||
| 10 | 1 | 2 | MPD | ||
| 11 | 1 | 2 | H2O | ||
| 2 | 1 | 1 | Na Cacodylate | ||
| 3 | 1 | 1 | KCl | ||
| 4 | 1 | 1 | spermine | ||
| 5 | 1 | 1 | MPD | ||
| 6 | 1 | 1 | H2O | ||
| 7 | 1 | 2 | MgCl2 | ||
| 8 | 1 | 2 | Na Cacodylate | ||
| 9 | 1 | 2 | KCl |
