3FAN
Crystal structure of chymotrypsin-like protease/proteinase (3CLSP/Nsp4) of porcine reproductive and respiratory syndrome virus (PRRSV)
Experimental procedure
Experimental method | MAD |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2007-03-15 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.5418, 0.9793, 0.9000 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 112.358, 49.162, 42.907 |
Unit cell angles | 90.00, 110.25, 90.00 |
Refinement procedure
Resolution | 27.910 - 1.900 |
R-factor | 0.19633 |
Rwork | 0.194 |
R-free | 0.24585 |
Structure solution method | MAD |
RMSD bond length | 0.017 |
RMSD bond angle | 1.572 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 32.300 | 1.980 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.045 | 0.167 |
Number of reflections | 17461 | |
<I/σ(I)> | 22.1 | 4.3 |
Redundancy | 4.5 | 4.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.3 | 291 | 0.1M sodium citrate pH 5.3, 0.7M ammonium phosphate monobasic, VAPOR DIFFUSION, HANGING DROP, temperature 291K |