3FA9
Crystal structure of the apo R132K:Y134F:R111L:L121D mutant of cellular retinoic acid-binding protein II at 1.94 angstrom resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-03-10 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0332 |
| Spacegroup name | P 1 |
| Unit cell lengths | 34.637, 37.173, 60.531 |
| Unit cell angles | 74.18, 73.24, 90.51 |
Refinement procedure
| Resolution | 19.360 - 1.940 |
| R-factor | 0.21573 |
| Rwork | 0.209 |
| R-free | 0.27598 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.091 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.560 | 2.030 |
| High resolution limit [Å] | 1.940 | 1.940 |
| Rmerge | 0.055 | 0.299 |
| Number of reflections | 22554 | |
| <I/σ(I)> | 12.3 | 2.2 |
| Completeness [%] | 87.9 | 88.4 |
| Redundancy | 1.9 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.1 | 298 | 0.1M BTP, 22% PEG4000, pH 8.1, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
