3FA7
Crystal structure of the apo R132K:R111L:L121E:R59E mutant of cellular retinoic acid-binding protein II at 1.90 angstrom resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-12-09 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97869 |
| Spacegroup name | P 1 |
| Unit cell lengths | 34.662, 37.212, 61.338 |
| Unit cell angles | 74.53, 73.39, 90.46 |
Refinement procedure
| Resolution | 35.710 - 1.900 |
| R-factor | 0.19207 |
| Rwork | 0.186 |
| R-free | 0.24909 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.648 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.073 | 0.368 |
| Number of reflections | 21661 | |
| <I/σ(I)> | 20.7 | 4.8 |
| Completeness [%] | 97.9 | 96.5 |
| Redundancy | 4 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 0.1M BTP, 22% PEG8000, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
