3FA6
Crystal structure of the R132K:Y134F:R111L:L121D:T54V mutant of cellular retinoic acid-binding protein II complexed with C15-aldehyde (a retinal analog) at 1.54 angstrom resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-08-08 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97869 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 37.261, 89.634, 90.621 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.160 - 1.540 |
| R-factor | 0.17388 |
| Rwork | 0.171 |
| R-free | 0.22055 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.623 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 63.760 | 1.600 |
| High resolution limit [Å] | 1.540 | 1.540 |
| Rmerge | 0.054 | 0.366 |
| Number of reflections | 45260 | |
| <I/σ(I)> | 31.3 | 2.5 |
| Completeness [%] | 98.7 | 96.7 |
| Redundancy | 4.6 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.8 | 277 | 0.1M sodium acetate, 18% PEG6000, pH 5.8, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
