3FA2
Crystal Structure of the BRCA1 Associated Ring Domain (BARD1) Tandem BRCT Domains
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2007-02-12 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.54178 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 44.979, 67.466, 86.783 |
Unit cell angles | 90.00, 99.20, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.200 |
R-factor | 0.22605 |
Rwork | 0.222 |
R-free | 0.29772 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2NTE CHAIN A |
RMSD bond length | 0.014 |
RMSD bond angle | 1.283 |
Data reduction software | d*TREK |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.080 |
High resolution limit [Å] | 2.010 | 2.010 |
Rmerge | 0.045 | 0.578 |
Number of reflections | 28268 | |
<I/σ(I)> | 16.77 | 1.22 |
Completeness [%] | 82.4 | 30.4 |
Redundancy | 3.3 | 2.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 0.1M MES 15% w/v PEG3350, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |