3FA0
Evaulaution at Atomic Resolution of the Role of Strain in Destabilizing the Temperature Sensitive T4 Lysozyme Mutant Arg96-->His
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-1 |
| Synchrotron site | SSRL |
| Beamline | BL9-1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-01-21 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 0.9800 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 60.370, 60.370, 96.570 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.000 - 1.090 |
| R-factor | 0.1576 |
| Rwork | 0.148 |
| R-free | 0.18830 |
| Structure solution method | AB INITIO |
| RMSD bond length | 0.015 |
| RMSD bond angle | 0.029 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELX |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.000 | 1.120 |
| High resolution limit [Å] | 1.090 | 1.090 |
| Rmerge | 0.570 | 0.350 |
| Number of reflections | 64093 | |
| <I/σ(I)> | 9.42 | 2.2 |
| Completeness [%] | 92.3 | 89.6 |
| Redundancy | 2.7 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.7 | 277 | 2 M Na/K phosphate, pH 6.7, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
