3F9V
Crystal Structure Of A Near Full-Length Archaeal MCM: Functional Insights For An AAA+ Hexameric Helicase
Experimental procedure
| Experimental method | SAD |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2007-10-29 |
| Detector | ADSC |
| Wavelength(s) | 0.97940 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 202.526, 202.526, 128.243 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 4.350 |
| R-factor | 0.415 |
| Rwork | 0.412 |
| R-free | 0.48100 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.195 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 4.350 |
| Number of reflections | 7760 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 291 | 50mM NaCl, 65mM CalCl2, 20% MPD, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
