3F9R
Crystal Structure of Trypanosoma Brucei phosphomannosemutase, TB.10.700.370
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E+ SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-11-06 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5408 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 105.927, 46.994, 94.494 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.850 |
| R-factor | 0.202 |
| Rwork | 0.199 |
| R-free | 0.26600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.471 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.122 | 0.862 |
| Number of reflections | 44877 | |
| <I/σ(I)> | 17.834 | 1.4 |
| Completeness [%] | 99.9 | 99.3 |
| Redundancy | 6.2 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 2.0 M (NH4)2SO4, 0.2 M NaCl, 0.1 M NaCacodylate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
