3F95
Crystal Structure of Extra C-terminal Domain (X) of Exo-1,3/1,4-beta-glucanase (ExoP) from Pseudoalteromonas sp. BB1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 113 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-07-13 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 73.416, 87.882, 56.459 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 21.750 - 1.800 |
| R-factor | 0.19055 |
| Rwork | 0.188 |
| R-free | 0.23391 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.635 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 21.750 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.046 | 0.279 |
| Number of reflections | 34484 | |
| <I/σ(I)> | 18.5 | 3.8 |
| Completeness [%] | 99.7 | 99.2 |
| Redundancy | 3.3 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 0.1M MES-NaOH, pH6.5, 25% PEG6000, 0.2M MgCl2, VAPOR DIFFUSION, HANGING DROP, temperature 291.0K |
