3F8U
Tapasin/ERp57 heterodimer
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-12-01 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9795 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 74.101, 72.295, 200.193 |
Unit cell angles | 90.00, 94.61, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.600 |
R-factor | 0.247 |
Rwork | 0.245 |
R-free | 0.28500 |
Structure solution method | SAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.400 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHARP |
Refinement software | CNS (1.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.690 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.074 | 0.207 |
Number of reflections | 63580 | |
<I/σ(I)> | 19.4 | 6.5 |
Completeness [%] | 97.4 | 91.4 |
Redundancy | 3.7 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 295 | reservoir contained 100 mM HEPES (@pH 7.5), 20% PEG 3K, 200 mM CaCl2. protein was concentrated to 10mg/ml in 150 mM NaCl, 20 mM Tris (@ pH 7.4), 5% glycerol. The hanging drop was supplemented to contain 100 mM guanidine HCl; seeding was used to obtain larger crystals., VAPOR DIFFUSION, HANGING DROP, temperature 295K |