3F8K

Crystal structure of protein acetyltransferase (PAT) from Sulfolobus solfataricus

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2006-07-03
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.97932, 0.97945, 0.94932
Spacegroup name	P 21 21 21
Unit cell lengths	44.701, 46.750, 68.624
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 1.840
R-factor	0.202
Rwork	0.202
R-free	0.22100
Structure solution method	MAD
RMSD bond length	0.070
RMSD bond angle	1.900
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.920
High resolution limit [Å]	1.840	1.840
Rmerge	0.068	0.423
Number of reflections	23521
<I/σ(I)>	23.1	2.5
Completeness [%]	98.6	89.2
Redundancy	5.9	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	300	12% PEG 20000, 0.05M MES pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 300K