3F8C
Crystal structure of multidrug binding transcriptional regulator LmrR complexed with Hoechst 33342
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-11-09 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.92 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 34.930, 34.930, 197.000 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.000 - 2.200 |
| R-factor | 0.212 |
| Rwork | 0.209 |
| R-free | 0.25800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3f8b |
| RMSD bond length | 0.013 |
| RMSD bond angle | 0.907 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.3.7) |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.270 | 2.300 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.220 | |
| Number of reflections | 6957 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6.8 | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9 | 298 | 25% PEG 1500, 0.1M succinic acid/phosphate/glycine (SPG buffer), pH 9.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
