3F84
Structure of fusion complex of major pilin CfaB and major pilin CfaB of CFA/I pilus from ETEC E. coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 75.151, 134.217, 65.040 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.350 |
| R-factor | 0.24356 |
| Rwork | 0.242 |
| R-free | 0.29392 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.377 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 15.000 |
| High resolution limit [Å] | 2.100 |
| Rmerge | 0.095 |
| Number of reflections | 32280 |
| <I/σ(I)> | 15.4 |
| Redundancy | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | The protein, 10 mg/ml in a buffer containing 20 mM Tris-HCl (pH 7.5) and 200 mM NaCl, was mixed in a 1:1 ratio with a well solution containing 30% PEG 8000 and 200 mM ammonium sulfate. VAPOR DIFFUSION |
