3F81
Interaction of VHR with SA3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E SUPERBRIGHT |
| Temperature [K] | 140 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-01-01 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.406, 59.186, 60.656 |
| Unit cell angles | 90.00, 98.36, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.900 |
| R-factor | 0.18046 |
| Rwork | 0.178 |
| R-free | 0.21901 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1vhr |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.724 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CCP4 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.950 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 26330 | |
| Completeness [%] | 99.1 | 99.7 |
| Redundancy | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 297 | 100 mM Tris 15% polyethylene glycol 8000, 60 mM NaF, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 297.0K |
