3F7Q
First pair of Fibronectin type III domains and part of the connecting segment of the integrin beta4
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | ENRAF-NONIUS FR591 |
Temperature [K] | 120 |
Detector technology | IMAGE PLATE |
Collection date | 2004-11-23 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 |
Unit cell lengths | 43.110, 49.470, 74.360 |
Unit cell angles | 107.22, 96.06, 106.60 |
Refinement procedure
Resolution | 22.272 - 1.750 |
R-factor | 0.1566 |
Rwork | 0.155 |
R-free | 0.18160 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1qg3 |
RMSD bond length | 0.005 |
RMSD bond angle | 0.878 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 69.480 | 1.810 |
High resolution limit [Å] | 1.750 | 1.750 |
Number of reflections | 50835 | |
<I/σ(I)> | 16.3 | 3.8 |
Completeness [%] | 91.6 | 84.8 |
Redundancy | 2.3 | 2.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7.5 | 298 | 0.1M Tris-HCl, 0.3M NaCl, 24% PEG 200, pH 7.5, VAPOR DIFFUSION, temperature 298K |