3F7D
SF-1 LBD bound by phosphatidylcholine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-06-25 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.1 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 46.895, 67.228, 82.689 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 23.760 - 2.200 |
R-factor | 0.182 |
Rwork | 0.180 |
R-free | 0.24100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ymt |
RMSD bond length | 0.008 |
RMSD bond angle | 1.006 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 2.280 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.070 | 0.240 |
Number of reflections | 13232 | |
<I/σ(I)> | 15.1 | 3.3 |
Completeness [%] | 95.9 | 89.5 |
Redundancy | 3.7 | 2.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 0.1 M sodium cacodylate, 20% PEG 3350, 0.2 M sodium acetate, 2 mM TCEP, 10% xylitol, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |