3F7A

Structure of Orthorhombic crystal form of Pseudomonas aeruginosa RssB

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2007-11-05
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97932
Spacegroup name	P 21 21 2
Unit cell lengths	120.873, 178.970, 57.067
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.307 - 4.308
R-factor	0.298
Rwork	0.296
R-free	0.34170
RMSD bond length	0.002
RMSD bond angle	0.556
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	4.450
High resolution limit [Å]	4.300	9.250	4.300
Rmerge	0.094	0.056	0.492
Number of reflections	8886
<I/σ(I)>	39.667
Completeness [%]	99.8	99.7	99.2
Redundancy	18.5	17.6	16.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		298	VAPOR DIFFUSION, temperature 298K