3F75
Activated Toxoplasma gondii cathepsin L (TgCPL) in complex with its propeptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-03-19 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.9800 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 65.621, 65.621, 149.772 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.980 - 1.990 |
| R-factor | 0.16739 |
| Rwork | 0.165 |
| R-free | 0.20557 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1CS8 (protease domain only) |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.156 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (refmac_5.5.0047) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.060 |
| High resolution limit [Å] | 1.990 | 1.990 |
| Rmerge | 0.122 | 0.744 |
| Number of reflections | 23266 | |
| <I/σ(I)> | 18.128 | 2.8 |
| Completeness [%] | 99.9 | 99.2 |
| Redundancy | 8.7 | 8.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4 | 298 | 40% PEG 8000, 0.1M Ammonium bromide, 0.1M Sodium citrate pH 4.0. Cryoprotected with ~10% Ethylene glycol, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
