3F6Z
Crystal structure of Pseudomonas aeruginosa MliC in complex with hen egg white lysozyme
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 4A |
| Synchrotron site | PAL/PLS |
| Beamline | 4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-07-10 |
| Detector | ADSC |
| Wavelength(s) | 1.000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 175.970, 90.960, 49.730 |
| Unit cell angles | 90.00, 98.17, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.500 |
| R-factor | 0.25 |
| Rwork | 0.238 |
| R-free | 0.27800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gpq |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.300 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.230 | 2.410 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.098 | 0.208 |
| Number of reflections | 34458 | |
| <I/σ(I)> | 18.6 | 8.5 |
| Completeness [%] | 1.0 | 100 |
| Redundancy | 6.8 | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 287 | 1.0M sodium citrate dehydrate, 0.1M HEPES (pH 7.5) , VAPOR DIFFUSION, HANGING DROP, temperature 287K |
