3F6T
Crystal structure of aspartate aminotransferase (E.C. 2.6.1.1) (YP_194538.1) from Lactobacillus acidophilus NCFM at 2.15 A resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-08-27 |
Detector | ADSC QUANTUM 315 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 89.780, 107.750, 119.680 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.921 - 2.150 |
R-factor | 0.18 |
Rwork | 0.177 |
R-free | 0.23800 |
Structure solution method | SAD |
RMSD bond length | 0.016 |
RMSD bond angle | 1.665 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SHELX |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 29.921 | 29.921 | 2.150 |
High resolution limit [Å] | 2.150 | 5.060 | 2.100 |
Rmerge | 0.147 | 0.055 | 0.805 |
Number of reflections | 63504 | ||
<I/σ(I)> | 9.204 | ||
Completeness [%] | 99.9 | 98.8 | 99.9 |
Redundancy | 3.6 | 3.6 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.9 | 277 | 0.2000M (NH4)2HPO4, 20.0000% PEG-3350, No Buffer pH 7.9, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K |