3F6O
Crystal structure of ArsR family transcriptional regulator, RHA00566
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-03-05 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9199 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 65.543, 65.543, 93.024 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 50.000 - 1.900 |
R-factor | 0.21692 |
Rwork | 0.216 |
R-free | 0.24693 |
Structure solution method | SAD |
RMSD bond length | 0.014 |
RMSD bond angle | 1.393 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.000 | 1.970 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.100 | 0.428 |
Number of reflections | 18153 | |
<I/σ(I)> | 9.8 | 2.8 |
Completeness [%] | 97.4 | 93.4 |
Redundancy | 9.1 | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 297 | 0.1M Bis-tris pH5.5 0.2M MgCl2 25%PEG3350 , VAPOR DIFFUSION, SITTING DROP, temperature 297K |