3F6N
Crystal structure of the virion-associated protein P3 from Caulimovirus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-02-15 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.931 |
| Spacegroup name | P 64 |
| Unit cell lengths | 104.943, 104.943, 72.527 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.850 - 3.100 |
| R-factor | 0.229 |
| Rwork | 0.222 |
| R-free | 0.28700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1t6f |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.037 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0053) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.270 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.065 | 0.306 |
| Number of reflections | 8369 | |
| <I/σ(I)> | 16.5 | 4.6 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 5.4 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 25% PEG 1000, 0.1M MES-NaOH buffer, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291.0K |
