3F6F

Crystal Structure of Glutathione Transferase dmGSTD10 from Drosophila melanogaster

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL13B1
Synchrotron site	NSRRC
Beamline	BL13B1
Temperature [K]	100
Detector technology	CCD
Collection date	2008-03-20
Detector	ADSC QUANTUM 315
Wavelength(s)	1
Spacegroup name	P 41 2 2
Unit cell lengths	49.649, 49.649, 195.373
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.230 - 1.600
R-factor	0.18681
Rwork	0.185
R-free	0.21855
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3f63
RMSD bond length	0.009
RMSD bond angle	1.155
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	AMoRE
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.660
High resolution limit [Å]	1.600	1.600
Number of reflections	33474
Completeness [%]	99.8	99.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	298	0.1M HEPES sodium pH 7.5, 0.8M Sodium phosphate monobasic monohydrate, 0.8M Potassium phosphate monobasic, VAPOR DIFFUSION, SITTING DROP, temperature 298.0K