3F5M

Crystal Structure of ATP-Bound Phosphofructokinase from Trypanosoma brucei

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX9.6
Synchrotron site	SRS
Beamline	PX9.6
Temperature [K]	100
Detector technology	CCD
Collection date	2002-09-02
Detector	ADSC QUANTUM 4
Wavelength(s)	0.896
Spacegroup name	P 21 21 21
Unit cell lengths	96.578, 117.570, 176.587
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.879 - 2.700
R-factor	0.2268
Rwork	0.224
R-free	0.28560
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2hig
RMSD bond length	0.009
RMSD bond angle	1.259
Refinement software	PHENIX ((phenix.refine))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.880	2.800
High resolution limit [Å]	2.700	2.700
Number of reflections	54525
<I/σ(I)>	9.2	2.3
Completeness [%]	97.9	97.8
Redundancy	4.3	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	298	2.3-2.7 M sodium formate, 0.1 M sodium acetate, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K