3F5D
Crystal Structure of a protein of unknown function from Bacillus subtilis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-10-29 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9795 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 75.597, 72.500, 47.560 |
| Unit cell angles | 90.00, 124.41, 90.00 |
Refinement procedure
| Resolution | 36.250 - 2.060 |
| R-factor | 0.213 |
| Rwork | 0.213 |
| R-free | 0.25700 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHARP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.130 |
| High resolution limit [Å] | 2.060 | 2.060 |
| Rmerge | 0.063 | 0.267 |
| Number of reflections | 12920 | |
| <I/σ(I)> | 28.8 | 2 |
| Completeness [%] | 96.5 | 83.9 |
| Redundancy | 4.2 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 0.1M HEPES 7.5, 20%PEG4000, 25% Iso-propanol, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
