3F5C
Structure of Dax-1:LRH-1 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-11-04 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 43 |
| Unit cell lengths | 103.355, 103.355, 117.455 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.785 - 3.000 |
| R-factor | 0.2236 |
| Rwork | 0.222 |
| R-free | 0.27920 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1pk5 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.253 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 3.100 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.079 | 0.360 |
| Number of reflections | 24366 | |
| <I/σ(I)> | 12.8 | 2 |
| Completeness [%] | 98.4 | 96.9 |
| Redundancy | 6 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 0.1 M HEPES, 20% PEG 8000, 1 mM Deoxy-BigChap, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298.0K |
