3F57
Crystal structure of human erythroid beta spectrin repeats 14 and 15 (ankyrin binding domain)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-G |
Synchrotron site | APS |
Beamline | 21-ID-G |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-03-17 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97857 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 65.330, 65.330, 288.900 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.840 - 2.900 |
R-factor | 0.26468 |
Rwork | 0.262 |
R-free | 0.32165 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.056 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | SHARP |
Refinement software | REFMAC (5.5.0056) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.840 | 3.030 |
High resolution limit [Å] | 2.900 | 2.900 |
Number of reflections | 14779 | |
Completeness [%] | 99.6 | 100 |
Redundancy | 7.2 | 7.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 295 | 30% PEG 3350, 0.2 M NaCl, 100 mM Tris-HCl pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |