3F52
Crystal structure of the clp gene regulator ClgR from C. glutamicum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-04-16 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0685 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 55.080, 55.080, 129.610 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.960 - 1.750 |
| R-factor | 0.18449 |
| Rwork | 0.183 |
| R-free | 0.21254 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3f51 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.361 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.000 | 1.850 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Number of reflections | 20938 | |
| <I/σ(I)> | 24.56 | 2.56 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 10 | 8.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 0.085 M HEPES, 8.5% PEG 8000, 15% glycerol, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
