3F4A
Structure of Ygr203w, a yeast protein tyrosine phosphatase of the Rhodanese family
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-06-27 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97929 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 74.382, 72.025, 53.514 |
Unit cell angles | 90.00, 103.60, 90.00 |
Refinement procedure
Resolution | 36.010 - 1.800 |
R-factor | 0.16808 |
Rwork | 0.166 |
R-free | 0.21064 |
Structure solution method | SAD |
Starting model (for MR) | none |
RMSD bond length | 0.016 |
RMSD bond angle | 1.564 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | SHELXCD |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.810 |
High resolution limit [Å] | 1.750 | 1.750 |
Rmerge | 0.080 | 0.295 |
Number of reflections | 27354 | |
<I/σ(I)> | 25.682 | 2.6 |
Completeness [%] | 98.7 | 89.3 |
Redundancy | 3.6 | 2.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 295 | 0.1 M Tris 8,5, 1.5 M LiSO4 plus 0.015 mg/ml trypsin. Cryoprotected with N-paratone oil, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 295K |