3F45
Structure of the R75A mutant of rat alpha-Parvalbumin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-10-18 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.542 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 27.315, 55.050, 28.768 |
| Unit cell angles | 90.00, 105.56, 90.00 |
Refinement procedure
| Resolution | 27.710 - 2.000 |
| R-factor | 0.14726 |
| Rwork | 0.144 |
| R-free | 0.20485 |
| Structure solution method | MIR |
| Starting model (for MR) | 1rtp |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.595 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | EPMR |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 27.710 |
| High resolution limit [Å] | 2.000 |
| Rmerge | 0.047 |
| Total number of observations | 20256 |
| Number of reflections | 5621 |
| Completeness [%] | 97.5 |
| Redundancy | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 290 | 3.2 Ammonium sulfate, 2% PEG 600, 100mM MES, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
