3F45
Structure of the R75A mutant of rat alpha-Parvalbumin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2005-10-18 |
Detector | MARRESEARCH |
Wavelength(s) | 1.542 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 27.315, 55.050, 28.768 |
Unit cell angles | 90.00, 105.56, 90.00 |
Refinement procedure
Resolution | 27.710 - 2.000 |
R-factor | 0.14726 |
Rwork | 0.144 |
R-free | 0.20485 |
Structure solution method | MIR |
Starting model (for MR) | 1rtp |
RMSD bond length | 0.016 |
RMSD bond angle | 1.595 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | EPMR |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 27.710 |
High resolution limit [Å] | 2.000 |
Rmerge | 0.047 |
Total number of observations | 20256 |
Number of reflections | 5621 |
Completeness [%] | 97.5 |
Redundancy | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 290 | 3.2 Ammonium sulfate, 2% PEG 600, 100mM MES, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 290K |