3F44
Crystal structure of putative monooxygenase (YP_193413.1) from Lactobacillus acidophilus NCFM at 1.55 A resolution
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-10-12 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.94645,0.97967 |
Spacegroup name | I 4 |
Unit cell lengths | 103.380, 103.380, 40.970 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 26.370 - 1.550 |
R-factor | 0.178 |
Rwork | 0.177 |
R-free | 0.19900 |
Structure solution method | MAD |
RMSD bond length | 0.017 |
RMSD bond angle | 1.584 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | SHELX |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 26.371 | 1.610 |
High resolution limit [Å] | 1.550 | 1.550 |
Rmerge | 0.065 | 0.619 |
Number of reflections | 31526 | |
<I/σ(I)> | 7.74 | 1.4 |
Completeness [%] | 98.1 | 96.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6 | 277 | 0.2000M Ca(OAc)2, 20.0000% PEG-8000, 0.1M MES pH 6.0, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K |