3F44
Crystal structure of putative monooxygenase (YP_193413.1) from Lactobacillus acidophilus NCFM at 1.55 A resolution
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-10-12 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.94645,0.97967 |
| Spacegroup name | I 4 |
| Unit cell lengths | 103.380, 103.380, 40.970 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 26.370 - 1.550 |
| R-factor | 0.178 |
| Rwork | 0.177 |
| R-free | 0.19900 |
| Structure solution method | MAD |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.584 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.371 | 1.610 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.065 | 0.619 |
| Number of reflections | 31526 | |
| <I/σ(I)> | 7.74 | 1.4 |
| Completeness [%] | 98.1 | 96.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 277 | 0.2000M Ca(OAc)2, 20.0000% PEG-8000, 0.1M MES pH 6.0, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
