3F41
Structure of the tandemly repeated protein tyrosine phosphatase like phytase from Mitsuokella multacida
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-09-21 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.2150,1.2146,1.1579 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 74.391, 73.304, 161.310 |
| Unit cell angles | 90.00, 93.71, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.300 |
| R-factor | 0.23 |
| Rwork | 0.208 |
| R-free | 0.25400 |
| Structure solution method | MAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.462 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.2.25) |
| Phasing software | SOLVE (2.12) |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 66.000 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.088 | 0.293 |
| Total number of observations | 28896 | |
| <I/σ(I)> | 2.2 | |
| Completeness [%] | 96.5 | 91.4 |
| Redundancy | 3.2 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8.5 | 298 | 10% PEG 8000, 2% ethylene glycol, 100 mM Tris, pH 8.5, vapor diffusion, temperature 298 K, VAPOR DIFFUSION |
