3F40

Crystal structure of NTF2-like protein of unknown function (YP_677363.1) from CYTOPHAGA HUTCHINSONII ATCC 33406 at 1.27 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2008-08-10
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.97936,0.91162,0.97925
Spacegroup name	C 2 2 21
Unit cell lengths	58.720, 60.280, 63.330
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.217 - 1.270
R-factor	0.183
Rwork	0.182
R-free	0.19900
Structure solution method	MAD
RMSD bond length	0.015
RMSD bond angle	1.519
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELX
Refinement software	REFMAC (5.4.0067)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	27.217	27.217	1.320
High resolution limit [Å]	1.270	2.730	1.270
Rmerge	0.056	0.044	0.519
Number of reflections	29394	5072	6098
<I/σ(I)>	8.27	18.9	1.9
Completeness [%]	94.9	87.7	97.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	277	0.2000M MgCl2, 30.0000% PEG-4000, 0.1M TRIS pH 8.5, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K