3F3X

Crystal structure of the transcriptional regulator BldR from Sulfolobus Solfataricus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ELETTRA BEAMLINE 5.2R
Synchrotron site	ELETTRA
Beamline	5.2R
Temperature [K]	100
Detector technology	CCD
Collection date	2006-05-20
Detector	MAR CCD 165 mm
Wavelength(s)	1.2
Spacegroup name	P 43 21 2
Unit cell lengths	86.110, 86.110, 43.240
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 1.900
Rwork	0.201
R-free	0.23000
Structure solution method	MAD
RMSD bond length	0.008
RMSD bond angle	1.200
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.970
High resolution limit [Å]	1.900	1.900
Number of reflections	13332
<I/σ(I)>	31.7	6.2
Completeness [%]	100.0	99.9
Redundancy	8.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.8	293	2.0M Ammonium sulphate, 0.1M Hepes, pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	7.8	293	2.0M Ammonium sulphate, 0.1M Hepes, pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K