3F3S
The Crystal Structure of Human Lambda-Crystallin, CRYL1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-10-04 |
| Detector | MAR225 |
| Wavelength(s) | 0.99987 |
| Spacegroup name | P 65 |
| Unit cell lengths | 102.085, 102.085, 134.986 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.730 - 2.000 |
| R-factor | 0.15654 |
| Rwork | 0.154 |
| R-free | 0.19629 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2DPO |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.463 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0055) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.730 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.128 | 1.297 |
| Number of reflections | 53793 | |
| <I/σ(I)> | 11.7 | 2.5 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 8.9 | 8.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 2M (NH4)2SO4, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
