3F3K
The structure of uncharacterized protein YKR043C from Saccharomyces cerevisiae.
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-06-29 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97948,0.97931 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 84.037, 86.277, 100.168 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.150 - 1.750 |
| R-factor | 0.143 |
| Rwork | 0.141 |
| R-free | 0.17000 |
| Structure solution method | MAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.346 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.780 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.074 | 0.265 |
| Number of reflections | 72754 | |
| <I/σ(I)> | 40.5 | |
| Completeness [%] | 98.2 | 98.4 |
| Redundancy | 9.9 | 8.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 0.2M Li3Citrate, 16% PEG 3350, 4%MPD, 10% Glycerol, Trypsin 1/10, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
