3F3H
Crystal structure and anti-tumor activity of LZ-8 from the fungus Ganoderma lucidium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-09-10 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.54179 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 33.190, 86.990, 92.130 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.100 |
| R-factor | 0.22218 |
| Rwork | 0.221 |
| R-free | 0.24552 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1osy |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.054 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.210 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.125 | 0.302 |
| Number of reflections | 15951 | |
| Completeness [%] | 98.0 | 93.6 |
| Redundancy | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 289 | 0.1M Tris, 1.75M ammonium sulfate, 6.4% polyethylene glycol 400, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 289.0K |
