3F3F
Crystal structure of the nucleoporin pair Nup85-Seh1, space group P21
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-05-08 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 79.840, 166.187, 188.904 |
Unit cell angles | 90.00, 93.02, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.900 |
R-factor | 0.246 |
Rwork | 0.246 |
R-free | 0.26500 |
Structure solution method | MIRAS |
RMSD bond length | 0.009 |
RMSD bond angle | 1.600 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SHARP |
Refinement software | CNS (1.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.970 |
High resolution limit [Å] | 2.900 | 2.900 |
Number of reflections | 108644 | |
<I/σ(I)> | 19.9 | 1.8 |
Completeness [%] | 97.7 | 78 |
Redundancy | 6.7 | 5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.1 | 298 | PEG 10000, MES buffer, pH 6.1, VAPOR DIFFUSION, HANGING DROP, temperature 298K |