Loading
PDBj
MenuPDBj@FacebookPDBj@TwitterPDBj@YouTubewwPDB FoundationwwPDB
RCSB PDBPDBeBMRBAdv. SearchSearch help

3F3F

Crystal structure of the nucleoporin pair Nup85-Seh1, space group P21

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsALS BEAMLINE 8.2.2
Synchrotron siteALS
Beamline8.2.2
Temperature [K]100
Detector technologyCCD
Collection date2008-05-08
DetectorADSC QUANTUM 315
Wavelength(s)1.0000
Spacegroup nameP 1 21 1
Unit cell lengths79.840, 166.187, 188.904
Unit cell angles90.00, 93.02, 90.00
Refinement procedure
Resolution50.000 - 2.900
R-factor0.246
Rwork0.246
R-free0.26500
Structure solution methodMIRAS
RMSD bond length0.009
RMSD bond angle1.600
Data reduction softwareHKL-2000
Data scaling softwareSCALEPACK
Phasing softwareSHARP
Refinement softwareCNS (1.2)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]50.0002.970
High resolution limit [Å]2.9002.900
Number of reflections108644
<I/σ(I)>19.91.8
Completeness [%]97.778
Redundancy6.75
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP6.1298PEG 10000, MES buffer, pH 6.1, VAPOR DIFFUSION, HANGING DROP, temperature 298K

227111

PDB entries from 2024-11-06

PDB statisticsPDBj update infoContact PDBjnumon